Pattern Magic

Product Information

You can create a garment by cutting, moving and reassemblingthe piecesof a pattern, just like the pieces of. a pazzle.

Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T (2006) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol 2: 2006 0007 10.1038/msb4100049 [ PMC free article] [ PubMed] [ Google Scholar] The companion volume to the cult Japanese Pattern Magic and Pattern Magic 2 that fans have been waiting for. Mutants of the remaining 58 ORFs (33 with mixtures and 25 with duplications; Figure 1C) were tested in a second set of PCR reactions, which was carried out using external primers flanking the targeted gene ( Figure 1A and B). A positive result in the first PCR test can occur not only from mutants with a partial duplication but also from ones that have been cross-contaminated from a nearby microplate well. Therefore, the second set of PCR tests was performed on three colonies after colony purification. In the second PCR test, colonies with the correct deletion or from a cross-contaminant mutant were expected to yield a single PCR product of length corresponding to the expected structure of the respective single-gene mutant or the structure of the targeted gene, respectively. In contrast, mutants with both the respective single-gene deletion and a genetic duplication were expected to yield both PCR products. In cases wherein the sizes of the predicted PCR products were indistinguishable for the deletion and wild-type structures, the PCR products were digested with XbaI before size separation by electrophoresis, which cuts within the kan (kanamycin resistance gene) replacement gene. The design of the Keio deletions was based on annotations that are now outdated. Of 4288 ORFs targeted, mutants were obtained for 3985 ( Baba et al, 2006). Re-annotation based on highly accurate sequencing of E. coli K-12 ( Hayashi et al, 2006) led to changing many coding regions and the total number of ORFs to 4296, including pseudogenes ( Riley et al, 2006) ( Supplementary Table I). The recent E. coli K-12 MG1655 GenBank record (U0096, released in December 2008) has an additional 97 ORFs (exclusive of the ORFs in IS elements, Supplementary Table II) that were not targeted. Of these 4214 annotated ORFs, 4186 were targeted for deletion and 28 were not ( Supplementary Table III), which resulted in the isolation of two independent mutants for 3864 targeted ORFs. No deletion was found for 299 ORFs, which are candidates for essential genes. Deletions were also isolated for 23 other ORFs; however, re-annotation led to re-classification of these ORFs as ‘split ORFs', because their coding regions are interrupted by an IS element or some other mutation ( Supplementary Table IV).bFraction of correct single-gene deletion candidates upon Keio collection construction ( Baba et al, 2006). Take the pieces of a three-dimensional garment apart and flatten them, to get individual pattern pieces. It’s a fairly well known series of patternmaking books from Japan. There are others, too, but what I love about these in particular, is that instead of just giving you the finished pattern to copy, the book explains how to draft it yourself! That way you have the possibility to make all kinds of different versions of your own using the various techniques explained. urn:oclc:record:1359080886 Foldoutcount 0 Identifier patternmagic20000naka Identifier-ark ark:/13960/s2cq6tmqwb4 Invoice 1652 Isbn 9781856697064 aEssentiality from PEC (Profiling of E. coli chromosome database, http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp).

Then there’s a design called ” Like a jungle” where the bodice front is separated with stylelines into slices that overlap each other. I had an idea that I wanted the overlapping area to create a flower-shape in the middle by leaving little holes in between the slices. Well, I’m not too happy with the result, but probably I could make it work by editing the pattern some more. Jungle-flowerHow about you? Do you have the Pattern Magic -books? Have you made, or planned, any garments with the patterns? Links: It’s difficult to say which one is my favorite. Each book has designs that I like. However I’ve done most variations on the pieces from book number 2, so I guess this means that it’s the most inspirational for me. What is Pattern Magic?

Ffareconceafed in a curve Thisdesignmakesyou wonderhow the flarehas beenconcealed. The foldedand layeredlook that hasso much depth is structurallybeautifuland exciting.With that ideain mind, I beganby drawing somecomplexcuryes. Ocr tesseract 5.2.0-1-gc42a Ocr_detected_lang en Ocr_detected_lang_conf 1.0000 Ocr_detected_script Latin Ocr_detected_script_conf 1.0000 Ocr_module_version 0.0.18 Ocr_parameters -l eng Old_pallet IA-NS-0001590 Openlibrary_edition

by cutting and opening out the pattern, but a flare that is subtly concealed on the underside of the fabric. Making a pattern Íor the kakurenbo design with a drawing is easier than you think.

The Keio collection ( Baba et al, 2006) has been established as a set of single-gene deletion mutants of Escherichia coli K-12. These mutants have a precisely designed deletion from the second codon from the seventh to the last codon of each predicted ORF. Further information is available at http://sal.cs.purdue.edu:8097/GB7/index.jsp or http://ecoli.naist.jp/. The distribution is now being handled by the National Institute of Genetics of Japan ( http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp). To date more than 4 million samples have been distributed worldwide. As we described earlier ( Baba et al, 2006), gene amplification during construction is likely to have led to a small number of mutants with genetic duplications. The relationship between the flat pattern pieces and the three-dimensional structure of the garment never changes. Easy-to-follow, detailed instructions make it easy to create stunning, sculptural clothes with a couture look There isn’t much information to be found about the author Tomoko Nakamichi. I searched on the internet, but I could only find the same information that’s also written in the Pattern Magic -books: She was a professor at Bunka Fashion College in Tokyo and now delivers lectures and holds courses both in Japan and internationally. All the garments in the pictures are actually in half-scale. Nakamichi created them with trial-and-error approach and they served to help her students understand better how patterns work. Basics

All the projects include step-by-step instructions with diagrams and lots of photos. Some are more difficult than others to understand, depending on your experience. Personally, I often use the books as inspiration, even just by looking at the pictures. If you've mastered the basics of pattern cutting, have caught the bug and are eager to experiment with complex 3d designs, you should definitely take a look at Pattern Magic' Tilly And The Buttons Blog