Roland Cy-5 Dual Trigger Cymbal Pad, 10In

Product Information

Though originally tested onboard Luda class missile destroyers and Jiangwei class missile frigates, the CY-1 can be carried by any surface combatant with C-801/802/803 launchers, from which the CY-1 can be launched, thus increasing the versatility and reducing the cost. In addition, a version is further modified so that it can be launched from torpedo tubes of submarines like the C-801, but there is not any confirmation that this version has entered the service. In an effort to boost possible export, the CY-1 has also been modified to carrying a various range of light torpedoes, such as that of USA, Italy, and Russian. However, there is no known export as of 2007. The CY-1 is also known to have been tested on the Type 039 submarine. Unlü M, Morgan ME, Minden JS (Oct 1997). "Difference gel electrophoresis: a single gel method for detecting changes in protein extracts". Electrophoresis. 18 (11): 2071–7. doi: 10.1002/elps.1150181133. PMID 9420172. S2CID 604007. requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available Alexa Fluor dyes, Dylight, FluoProbes dyes, Sulfo Cy dyes, [13] Seta dyes, [14] IRIS dyes from Cyanine Technologies [15] and others can be used interchangeably with Cy dyes in most biochemical applications, with claimed improvements in solubility, fluorescence, or photostability. [16] [17]

Harvey, B. J., Perez, C. & Levitus, M. DNA sequence-dependent enhancement of Cy3 fluorescence. Photochem. Photobiol. Sci. 8, 1105–1110 (2009). The design of DNA oligonucleotides is based on a permutation scheme allowing for all possible combinations of 5 consecutive nucleotides immediately adjacent to a fluorescent dye to be synthesized in parallel as microarrays (P 1–P 5 in Fig. 1). The total amount of unique pentanucleotides is 4 5, or 1024 oligonucleotides to be synthesized. To account for potential variability in synthesis efficiency which would produce lower fluorescence signals for poorly-synthesized sequences, the nucleotide content in all DNA sequences is kept constant by adding a subsection composed of five “N” trinucleotides (N 1 to N 5) where each N x corresponds to AGCT minus the nucleotide in P x. Between the P and N sections, a T 15 spacer is introduced and the entire oligonucleotide sequence is then synthesized over a T 5 linker separating the DNA from the surface of the array. At the 3′ end of the oligonucleotide, immediately after the pentanucleotide, a Cy3 or Cy5 dye is attached. Schematically, all 1024 combinations are represented in the form 3′Cy3/Cy5—P 1P 2P 3P 4P 5—T 15—(ACGT-P 1)—(ACGT-P 2)—(ACGT-P 3)—(ACGT-P 4)—(ACGT-P 5)—T 5—glass. Microarray synthesisHolz, K. et al. High-efficiency reverse (5’–>3’) synthesis of complex DNA microarrays. Sci. Rep. 8, 15099 (2018). Fuller, C. W. et al. The challenges of sequencing by synthesis. Nat. Biotechnol. 27, 1013–1023 (2009). Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries. Iris Dyes | Cyanine Technologies". Archived from the original on 2015-01-26 . Retrieved 2015-01-26.

are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, Kaur, G., Lewis, J.S. & van Oijen, A.M. Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA. Molecules 24 (2019).commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment. Sanborn, M. E., Connolly, B. K., Gurunathan, K. & Levitus, M. Fluorescence properties and photophysics of the sulfoindocyanine Cy3 linked covalently to DNA. J. Phys. Chem. B 111, 11064–11074 (2007). Norman, D. G., Grainger, R. J., Uhrin, D. & Lilley, D. M. J. Location of cyanine-3 on double-stranded DNA: Importance for fluorescence resonance energy transfer studies. Biochemistry 39, 6317–6324 (2000). Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: Flow cytometry, Microscopy (mainly Visible range, but also UV, IR), Microplate assays, Microarrays, as well as "light-up Probes," and in vivo imaging. [18] Nucleic acid labeling [ edit ]

Use of this material to perform services for a fee for third parties, including contract research and drug screening. Nizamov, T. R.; Iliasov, A. R.; Vodopyanov, S. S.; Kozhina, I. V.; Bordyuzhin, I. G.; Zhukov, D. G.; Ivanova, A. V.; Permyakova, E. S.; Mogilnikov, P. S.; Vishnevskiy, D. A.; Shchetinin, I. V.; Abakumov, M. A.; Savchenko, A. G. Study of Cytotoxicity and Internalization of Redox-Responsive Iron Oxide Nanoparticles on PC-3 and 4T1 Cancer Cell Lines. Pharmaceutics, 2023, 15(1), 127. doi: 10.3390/pharmaceutics15010127 documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products orCy3 and Cy5 are used in proteomics experiments so that samples from two sources can be mixed and run together through the separation process. [19] [20] This eliminates variations due to differing experimental conditions that are inevitable if the samples were run separately. These variations make it extremely difficult, if not impossible, to use computers to automate the acquisition of the data after the separation is complete. Using these dyes makes the automation trivial. The Cy3 and Cy5 nomenclature was first proposed by Ernst, et al. [5] in 1989, and is non-standard since it gives no hint of their chemical structures. In the original paper the number designated the count of the methines (as shown), and the side chains were unspecified. Due to this ambiguity various structures are designated Cy3 and Cy5 in the literature. The R groups do not have to be identical. In the dyes as used they are short aliphatic chains one or both of which ends in a highly reactive moiety such as N-hydroxysuccinimide or maleimide. The main application for cyanine dyes is in biological labeling. Nevertheless, there is a wide literature on both their synthesis and uses, and cyanines are common in some CD and DVD media. Levitus, M. & Ranjit, S. Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments. Q Rev. Biophys. 44, 123–151 (2011).

Sale, lease, license or other transfer of the material or any material derived or produced from it. This Cyanine5 NHS ester (analog to Cy5 ® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties. a b Mujumdar B, Ernst A, Mujumdar SR, Lewis CJ, Waggoner AS (Mar 1993). "Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters". Bioconjugate Chemistry. 4 (2): 105–111. doi: 10.1021/bc00020a001. PMID 7873641. Example: assuming the required marker protein is 500 μL 2 mg/mL IgG (MW=150,000), use 100 μL DMSO dissolve 1 mg Cy5.5, the required Cy5.5 volume is 5.05 μL, and the detailed calculation process is as follows: CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.Agbavwe, C. et al. Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays. J. Nanobiotechnol. 9 (2011). Holz, K., Lietard, J. & Somoza, M. M. High-power 365 nm UV LED mercury arc lamp replacement for photochemistry and chemical photolithography. ACS Sustain. Chem. Eng. 5, 828–834 (2017). Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.