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Song J, Wu Z, Wang Z, Deng S, Zhou S. Kruppel-homolog 1 mediates juvenile hormone action to promote vitellogenesis and oocyte maturation in the migr

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I.N.]] [[I.O.]] [[I.P.]] [[I.Q.]] [[I.R.]] [[I.S.]] [[I.T.]] [[I.U.]] [[I.V.]] [[I.W.]] [[I.X.]] [[I.Y.]] [[I.Z.]] Urena E, Manjon C, Franch-Marro X, Martin D. Transcription factor E93 specifies adult metamorphosis in hemimetabolous and holometabolous insects. P Natl Acad Sci USA. 2014;111(19):7024–9. R.N.]] [[R.O.]] [[R.P.]] [[R.Q.]] [[R.R.]] [[R.S.]] [[R.T.]] [[R.U.]] [[R.V.]] [[R.W.]] [[R.X.]] [[R.Y.]] [[R.Z.]]

x.a.]] [[x.b.]] [[x.c.]] [[x.d.]] [[x.e.]] [[x.f.]] [[x.g.]] [[x.h.]] [[x.i.]] [[x.j.]] [[x.k.]] [[x.l.]] [[x.m.]] M.n.]] [[M.o.]] [[M.p.]] [[M.q.]] [[M.r.]] [[M.s.]] [[M.t.]] [[M.u.]] [[M.v.]] [[M.w.]] [[M.x.]] [[M.y.]] [[M.z.]] D.A.]] [[D.B.]] [[D.C.]] [[D.D.]] [[D.E.]] [[D.F.]] [[D.G.]] [[D.H.]] [[D.I.]] [[D.J.]] [[D.K.]] [[D.L.]] [[D.M.]] dA]] [[dB]] [[dC]] [[dD]] [[dE]] [[dF]] [[dG]] [[dH]] [[dI]] [[dJ]] [[dK]] [[dL]] [[dM]] [[dN]] [[dO]] [[dP]] [[dQ]] [[dR]] [[dS]] [[dT]] [[dU]] [[dV]] [[dW]] [[dX]] [[dY]] [[dZ]]

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k.n.]] [[k.o.]] [[k.p.]] [[k.q.]] [[k.r.]] [[k.s.]] [[k.t.]] [[k.u.]] [[k.v.]] [[k.w.]] [[k.x.]] [[k.y.]] [[k.z.]] Za.]] [[Zb.]] [[Zc.]] [[Zd.]] [[Ze.]] [[Zf.]] [[Zg.]] [[Zh.]] [[Zi.]] [[Zj.]] [[Zk.]] [[Zl.]] [[Zm.]] B.A.]] [[B.B.]] [[B.C.]] [[B.D.]] [[B.E.]] [[B.F.]] [[B.G.]] [[B.H.]] [[B.I.]] [[B.J.]] [[B.K.]] [[B.L.]] [[B.M.]]P.a.]] [[P.b.]] [[P.c.]] [[P.d.]] [[P.e.]] [[P.f.]] [[P.g.]] [[P.h.]] [[P.i.]] [[P.j.]] [[P.k.]] [[P.l.]] [[P.m.]] e.a.]] [[e.b.]] [[e.c.]] [[e.d.]] [[e.e.]] [[e.f.]] [[e.g.]] [[e.h.]] [[e.i.]] [[e.j.]] [[e.k.]] [[e.l.]] [[e.m.]] f.n.]] [[f.o.]] [[f.p.]] [[f.q.]] [[f.r.]] [[f.s.]] [[f.t.]] [[f.u.]] [[f.v.]] [[f.w.]] [[f.x.]] [[f.y.]] [[f.z.]]

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ga]] [[gb]] [[gc]] [[gd]] [[ge]] [[gf]] [[gg]] [[gh]] [[gi]] [[gj]] [[gk]] [[gl]] [[gm]] [[gn]] [[go]] [[gp]] [[gq]] [[gr]] [[gs]] [[gt]] [[gu]] [[gv]] [[gw]] [[gx]] [[gy]] [[gz]] gn.]] [[go.]] [[gp.]] [[gq.]] [[gr.]] [[gs.]] [[gt.]] [[gu.]] [[gv.]] [[gw.]] [[gx.]] [[gy.]] [[gz.]] Belles X, Santos CG. The MEKRE93 (Methoprene tolerant-Kruppel homolog 1-E93) pathway in the regulation of insect metamorphosis, and the homology of the pupal stage. Insect Biochem Mol Biol. 2014;52:60–8. sA]] [[sB]] [[sC]] [[sD]] [[sE]] [[sF]] [[sG]] [[sH]] [[sI]] [[sJ]] [[sK]] [[sL]] [[sM]] [[sN]] [[sO]] [[sP]] [[sQ]] [[sR]] [[sS]] [[sT]] [[sU]] [[sV]] [[sW]] [[sX]] [[sY]] [[sZ]]MN.]] [[MO.]] [[MP.]] [[MQ.]] [[MR.]] [[MS.]] [[MT.]] [[MU.]] [[MV.]] [[MW.]] [[MX.]] [[MY.]] [[MZ.]] U.N.]] [[U.O.]] [[U.P.]] [[U.Q.]] [[U.R.]] [[U.S.]] [[U.T.]] [[U.U.]] [[U.V.]] [[U.W.]] [[U.X.]] [[U.Y.]] [[U.Z.]] Total RNA was extracted from the ovary, CA, epidermis, midgut, colleterial glands, fat body, or brain using TRIzol™ Reagent (Invitrogen, MA, USA). Total RNA (2 μg) was reverse transcribed into cDNA using a SMARTer® PCR cDNA Synthesis Kit (Takara, Dalian, China), following the manufacturer’s instructions. qPCR was performed using Hieff® qPCR SYBR Green Master Mix (Low Rox Plus) (Yeasen Biotech, Shanghai, China) and Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, MA, USA). The thermocycling conditions were as follows: 94 °C for 2 min, 40 cycles of 94°C for 10 s, and 56 °C for 30 s. Actin-5c was used as an internal reference [ 76]. The relative expression levels of the indicated genes were computed using the 2-ΔΔCt method [ 25, 76]. The primer sequences used for qPCR are listed in Table S1 (Additional file 1). JH III extraction and quantification