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Porreca, Gregory J (2010). "Genome sequencing on nanoballs". Nature Biotechnology. 28 (1): 43–4. doi: 10.1038/nbt0110-43. PMID 20062041. S2CID 54557996. An avid player, Chris currently maintains a handicap index of 2.4 and has a career-low round of 66, which he has shot on three occasions. He lives about 20 miles north of Atlanta in Roswell, Georgia, with his wife, Stacey, and is a member at Atlanta National Golf Club. DNA Nanoball Sequencing involves isolating DNA that is to be sequenced, shearing it into small 100 – 350 base pair (bp) fragments, ligating adapter sequences to the fragments, and circularizing the fragments. The circular fragments are copied by rolling circle replication resulting in many single-stranded copies of each fragment. The DNA copies concatenate head to tail in a long strand, and are compacted into a DNA nanoball. The nanoballs are then adsorbed onto a sequencing flow cell. The color of the fluorescence at each interrogated position is recorded through a high-resolution camera. Bioinformatics are used to analyze the fluorescence data and make a base call, and for mapping or quantifying the 50bp, 100bp, or 150bp single- or paired-end reads. [6] [2] DNA Isolation, fragmentation, and size capture [ edit ] Another advantage of DNA nanoball sequencing include the use of high-fidelity Phi 29 DNA polymerase [10] to ensure accurate amplification of the circular template, several hundred copies of the circular template compacted into a small area resulting in an intense signal, and attachment of the fluorophore to the probe at a long distance from the ligation point results in improved ligation. [2] Disadvantages [ edit ]

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The best premium golf balls come in alternative versions that spin slightly less for players who prefer a firmer feel or more control off the tee. We recommend you try both kinds of feel from various distances to find your preferred feel. The FISSEQ technology has been licensed to ReadCoor Inc., a start up company spun out of the Wyss Institute, which will commercialize it as a new generation sequencing platform, allowing researchers to perform high throughput RNA sequencing and obtain the cellular locations of multiple RNAs simultaneously in intact cell and tissue samples of their choice. An updated reference human genome dataset of the BGISEQ-500 sequencer". GigaDB . Retrieved 22 March 2017. a b Blanco, Luis; Bernad, Antonio; Lázaro, José M.; Martin, Gil; Garmendia, Cristina; Margarita, M; Salas (1989). "Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication". The Journal of Biological Chemistry. 264 (15): 8935–40. doi: 10.1016/S0021-9258(18)81883-X. PMID 2498321.As its name might suggest, the Wilson Duo Soft+ is the softest golf ball in the current Wilson range. In fact, its compression rating of 35 is among the lowest of any golf ball that’s been manufactured in recent years. The Duo Soft+ features a two-piece construction and a surlyn cover. That said, while surlyn is significantly less responsive than urethane, which is also far more expensive, the Duo Soft+ provides better greenside control than what players might normally expect from a two-piece ball.

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Fullwood, M. J.; Wei, C.-L.; Liu, E. T.; Ruan, Y. (2009). "Next-generation DNA sequencing of paired-end tags (PET) for transcriptome and genome analyses". Genome Research. 19 (4): 521–32. doi: 10.1101/gr.074906.107. PMC 3807531. PMID 19339662. DNA nanoball sequencing technology offers some advantages over other sequencing platforms. One advantage is the eradication of optical duplicates. DNA nanoballs remain in place on the patterned array and do not interfere with neighboring nanoballs. FFFFFFFFFFFGFGFFFFFF;FFFFFFFGFGFGFFFFFF;FFFFGFGFGFFEFFFFFEDGFDFF@FCFGFGCFFFFFEFFEGDFDFFFFFGDAFFEFGFFPerhaps the most interesting golf ball in Wilson's current lineup is the new Staff Model R. From a construction standpoint, it mirrors the Staff Model and is Wilson’s second premium golf ball offering, designed for Tour-quality distances and feel. Like the Staff Model, the Staff Model R features a four-piece construction and Wilson’s V-COR core, which combine to produce explosive distances in the long game. It also delivers the control and feel that golfers want with their scoring clubs on full shots and around the green. Here at Golf Monthly, our highly experienced team of testers play a lot of golf in different conditions throughout the year. Through our rigorous testing procedures, our collective goal is to test every golf model on the market as soon as it becomes available, putting in the hours in all weathers out on the golf course, the practice ground and the short-game area. Chrisey, L.; Lee, GU; O'Ferrall, CE (1996). "Covalent attachment of synthetic DNA to self-assembled monolayer films". Nucleic Acids Research. 24 (15): 3031–9. doi: 10.1093/nar/24.15.3031. PMC 146042. PMID 8760890. Fehlmann, T. (2016). "cPAS-based sequencing on the BGISEQ-500 to explore small non-coding RNAs". Clin Epigenetics. 8: 123. doi: 10.1186/s13148-016-0287-1. PMC 5117531. PMID 27895807. Urethane-covered balls offer the best spin control and feel but do cost more. Experiment with each to see if you notice enough of a difference to justify the extra cost, or whether the best mid-price golf balls might be an option.

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Play George Church, Ph.D., a Core Faculty member at the Wyss Institute and Professor of Genetics at Harvard Medical School, explains how fluorescent in situ sequencing could lead to new diagnostics that spot the earliest signs of disease, and how it could help reveal how neurons in the brain connect and function. Credit: Wyss Institute at Harvard University. Which aspect of the game is most important to you? Are you looking for maximum distance or something that allows your short game to shine around the greens? The data generated from the DNA nanoballs is formatted as standard FASTQ formatted files with contiguous bases (no gaps). These files can be used in any data analysis pipeline that is configured to read single-end or paired-end FASTQ files. Revolocity™ Whole Genome Sequencing Technology Overview" (PDF). Complete Genomics . Retrieved 18 November 2017.Adapter DNA sequences must be attached to the unknown DNA fragment so that DNA segments with known sequences flank the unknown DNA. In the first round of adapter ligation, right (Ad153_right) and left (Ad153_left) adapters are attached to the right and left flanks of the fragmented DNA, and the DNA is amplified by PCR. A splint oligo then hybridizes to the ends of the fragments which are ligated to form a circle. An exonuclease is added to remove all remaining linear single-stranded and double-stranded DNA products. The result is a completed circular DNA template. [2] Rolling circle replication [ edit ] a b c d e f g h i j Drmanac, R.; Sparks, A. B.; Callow, M. J.; Halpern, A. L.; Burns, N. L.; Kermani, B. G.; Carnevali, P.; Nazarenko, I.; etal. (2009). "Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays". Science. 327 (5961): 78–81. Bibcode: 2010Sci...327...78D. doi: 10.1126/science.1181498. PMID 19892942. S2CID 17309571. Are you very much a classic white ball player, or someone open to playing something a little different as the golf ball world evolves? There's more choice than ever in this regard and if you are reading this guide, chances are you want a slightly different color or look to your golf ball. Wilson offers two premium golf balls in its current range, the Staff Model and Staff Model R, both of which offer tour-proven performance. Both balls feature a four-piece construction and highly responsive urethane cover, with the result being distance and control in the long game and ample spin and soft feel on and around the greens. The primary difference between the two models is that the R version of the ball is unpainted, which Wilson says will deliver a more controlled trajectory, increased short game spin, and improved accuracy on full shots. Do any professional golfers use Wilson golf balls? Publication March 8, 2021 Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses