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A diet rich in ‘superfoods’ that includes green veggies and fruits is essential for maintaining overall health. However, according to the Centers for Disease Control and Prevention (CDC), merely 9% of American adults meet the recommended intake of greens and fruits. 93% of Australian adults don’t eat the recommended five serves of vegetables and 50% don’t eat two serves of fruit. We created OxyGreens to be the MOST DELICIOUS natural, super greens on the market, enhanced with alkalizing forest berries and wholefood prebiotic fiber. Optimal stimulant dosage so you're not sitting there so full of energy, sweating and getting jittery Wild Melon: Natural Flavor, Silicon Dioxide, Calcium Silicate, Sucralose, DL-Malic Acid, Turmeric (Color), Sodium Chloride, Citric Acid, Fruit & Vegetable Juice Powder (Color)
Chromatographic screening method for dioxygenase mutein screening - An analytical chromatographic method based on the LC-MS technology, that enables semi-quantitative medium throughput analysis of product, substrate, and co-substrate (glucose and a-ketoglutarate) of the KGDO-reaction was developed. Furthermore a product-based, quantitative, colorimetric assay was adapted to 96-well plates and made suitable for high-throughput screening of the KGDO-reaction, allowing a screening for new KDGO-muteins in MTP format. The assay is very specific and highly sensitive, as it can detect down to 1 µM product in the linear range of 1 to 40 µM. With this new screening assay in hand the screening of KGDO-libraries generated in WP2 was performed and new muteins with desired properties were found. After cell lysis, the in-vitro screening procedure based on spectrophotometric determination of hydroxyproline was performed to identify optimized P4H variants from SeSAM library. Muteins with different kinetic characters were identified. By applying the same assay, the obtained muteins were characterized with regard to catalytic efficiency, whereas the industrial partner REX tested the new catalysts during up-scaling. P450 muteins active with conducting polymers - The screening of the structure-guided P450 BM3 mutant libraries did not lead to activity improved variants with a conducting polymer for the electrochemical conversions. This alternative cofactor system was identified to be not suitable for an electrochemical application therefore the research was focused on the second proposed alternative cofactor system: Mediated Electron Transfer (MET). Previous studies on MET build the proof of concept. The evolution of P450 BM3 M3 reported by Nazor et al. for improving the mediated electron transfer (MET) could further be extended towards increasing activity in presence of the alternative cofactor system Zn/Co(III)-sep. As starting P450 BM3 mutant was evolved in using epPCR and SeSaM for generation of random mutant libraries. The libraries were enriched for active clones with the new high throughput flow cytometer whole cell screening (FACS) system established in within the project, see below. The clones were screened for improved activity using Zn/Co(III)-sep as sole electron source for P450 activity. Improved variants were sequenced and characterized for key catalytic parameters. The best P450 BM3 mutant displayed a nearly 4 fold higher activity (kcat = 6.8 mmol product min-1 mmol-1 P450) compared to the starting variant P450 BM3 DM using the alternative Zn/Co(III)-sep cofactor system. In addition, the catalytic efficiency could be increased by 2.5 fold compared to the starting template. Four new hot spots could be identified, saturated individually and recombined to select the most beneficial amino acid combination for improved MET in P450 BM3. A further improved mutein was found with a 1.4 fold improved activity. Summarizing, the selected improved variants give valuable information, which amino acid substitutions are relevant for MET-based biocatalysis. Passionfruit: Natural Flavor, Sucralose, DL-Malic Acid, Silicon Dioxide, Citric Acid, Calcium Silicate, Turmeric (Color)EHP Labs have carefully selected Chicory Root (Inulin), Green Kiwi Fruit, Green Banana, and Larch as our powerful prebiotic fibre complex for our OxyGreens. These superfoods help to nourish the good gut bacteria (microbiome) and promote an overall healthy digestive system. This will improve nutrient absorption in the gut lining, as well as promote bowel regularity and reduce stomach bloating. You will feel lighter and more energetic. Every Day Convenient Health Cell-based screening methods -To generate designer enzymes by protein engineering comprises three subsequent steps: efficient generation of diversity on the protein level, efficient expression of the target enzyme in the expression host and an efficient screening system to identify novel enzymes with desired features. The availability of an appropriate screening system is essential in protein engineering experiments (“You get what you screen for!”) and is thus determining the success of such approaches. Many important redox enzymes need NAD based cofactors, which are efficiently regenerated by the cellular metabolism. In addition whole cells can also act as a cover protecting the enzymes from shear forces or organic solvents and, thus, increase their stability. On the other hand such the protecting cell sometimes limits substrate access to the intracellular enzymes. Another advantage of cell based screenings is that enzyme isolation and purification steps can be circumvented which speeds up the screening process. Therefore to speed up breeding cycles, to reduce costs and to simulate later processes as far as possible, work focused on the development of whole cell screening systems. Ilić T, Dodevska M, Marčetić M, Božić D, Kodranov I, Vidović B. Chemical Characterization, Antioxidant and Antimicrobial Properties of Goji Berries Cultivated in Serbia. Foods. 2020 [cited 2022 January 20];9(11):1614. DOI: 10.3390/foods9111614. Inulin, Organic Barley Grass, Organic Wheat Grass, Natural Flavours, Citric Acid, Larch (Arabinogalactan), Green Banana, Organic Kale, Organic Spinach, Organic Spirulina, Celery, Kiwi Fruit, Sweetener (Sucralose), Vegetable Gum (Acacia), Silicon Dioxide, Organic Acai, Organic Goji Fruit Extract, Potassium Chloride, Organic Chlorella, Blueberry, Calcium Silicate, Broccoli Seed Extract (13.5% Glucoraphanin), Kelp Laminaria.
The leading ingredients of our OxyGreens are Barley Grass, Broccoli, Kale, and Spinach. These powerful greens are rich in magnesium and vitamins A, K, E and C, which act as antioxidants and help support a healthy immune system. Antioxidants help the body remove unwanted toxins that can build up as a result of natural processes and environmental stressors we may experience in daily life. Additional greens we have added to our greens complex include Celery, Spirulina, Chlorella and Kelp, which are rich in vitamins, minerals, iron and chlorophyll (the stuff that makes leaves green) to help maintain a healthy immune system, healthy digestive function, and support overall wellbeing. Alkalising Berries with Vitamin C The phosphate-based screening assay has been described in two scientific publications and has already helped to identify interesting enzymes, see below. OxyGreens not only contains a green superfood complex but also antioxidants, prebiotic fiber and an array of other wellness ingredients in a convenient 1-scoop serving. The best part is all these benefits are delivered with an amazing taste. The goal of OXYGREEN is to gather knowledge on the functions of selected enzymes that use oxygen to perform industrially relevant reactions. This knowledge is intended to be applied for the development of enzyme tools of interest for industrial and biotechnological applications. The project is based on three main cornerstones: (i) biochemistry to understand enzyme function; (ii) genetics and microbiology to modify (‘engineering’ in technical jargon) the biocatalysts of interest as to perform the desired reactivities; and (iii) biotechnology and chemistry to develop the technical tools for the application and usage of the modified biocatalysts and make them attractive for end-user industrial applications. Inulin, Organic Barley Grass, Organic Wheat Grass, Larch (Arabinogalactan), Green Banana, Organic Kale, Organic Spinach, Organic Spirulina, DL-Malic Acid, Natural Flavours, Citric Acid, Celery, Kiwi Fruit, Sweetener (Sucralose), Silicon Dioxide, Organic Acai, Calcium Silicate, Organic Goji Fruit Extract, Organic Chlorella, Blueberry, Broccoli Seed Extract (13.5% Glucoraphanin), Kelp Laminaria.
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Guava Paradise:Natural Flavor, Citric Acid, Sucralose, Silicon Dioxide, Calcium Silicate, Beet & Carrot Juice Powder (Color), Sodium Chloride, Turmeric (Color)
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