HAZET 2250-3 31 mm Adapter - Chrome-Plated

Product Information

He H. H., Li L. Y., Liu Y., Kassymova M., Li D. D., Zhang L. L., Jiang H. L., Nano Res., 2021, 14, 444 Inhibition of glycolytic and TCA cycle‐related enzymes. (A) Recombinant human GAPDH treated with 100 and 250μM GP‐2250 for 30 and 60min, followed by GAPDH enzyme activity assay. (B) Inhibition of GAPDH activity in Panc Tul and BxPC3 cells following incubation with GP‐2250 (500μM) for 24h. The GAPDH inhibitor DMF, 100μM, was included as control. (C) Human recombinant hexokinase 2 (HK2) incubated with 250 and 500μM GP‐2250 for 60min. Formation of NADH was measured every 5min. (D) Inhibition of hexokinase 2 (HK2)activity in Panc Tul and BxPC3 cells following incubation with GP‐2250 (250, 500 and 1000μM) for 24h. (E) Inhibition of alpha‐ketoglutarate dehydrogenase (αKGDH) following treatment of Panc Tul and BxPC3 cells for 24h with GP‐2250 (250, 500 and 1000μM). (F) Inhibition of pyruvate dehydrogenase (PDH) following treatment of Panc Tul and BxPC3 cells for 24h with GP‐2250 (250 and 500μM). Concentrations of GP‐2250 ranging from 100 to 1000μM were used in the cellular assays of GAPDH, HK2, αKGDH and PDH, Shown are the results of the lowest effective concentrations, respectively. * p≤0.05, significant; ** p≤0.01, highly significant; *** p≤0.001, extremely significant. DMF, dimethylfumarate; GAPDH, glyceraldehyde‐3‐phosphate‐dehydrogenase; HK2, hexokinase 2; NADH, nicotinamide adenine dinucleotide+hydrogen; NC, negative control; U, untreated. Both HK2 and GAPDH are upregulated in many human tumours and serve as drug targets. 8 Inhibitors of HK2 such as benitrobenrazide show anticancer activity as demonstrated in tumour xenograft models 29 and clinical trials have started with the HK2 inhibitor lonidamine. 8 Apart from limiting glycolysis, HK2 inhibition is expected to contribute to the rise of ROS by reducing the synthesis of the reducing agent NADPH through inhibition of the pentose phosphate pathway. 5, 30

Orr K. W. P., Collins S. M., Reynolds E. M., Nightingale F., Bostroem H. L. B., Cassidy S. J., Dawson D. M., Ashbrook S. E., Magdysyuk O. V., Midgley P. A., Goodwin A. L., Yeung H. H. M., Chem. Sci., 2021, 12, 4494 Duke GJ, Barker A, Rasekaba T, et al. Development and validation of the critical care outcome prediction equation, version 4. Crit Care Resusc. 2013;15:191–7. Fan Z. Y., Staiger L., Hemmer K., Wang Z., Wang W. J., Xie Q. J., Zhang L. J., Urstoeger A., Schuster M., Lercher J. A., Cokoja M., Fischer R. A., Cell Rep. Phys. Sci., 2022, 3, 100757 De Boni L, Piovesan E, Misoguti L, Zílio SC, Mendonca CR (2007) Two-photon absorption dependence on the temperature for Azoaromatic compounds: effect of molecular conformation. J Phys Chem A 111:6222–6224 The mTOR complex 1 (mTORC1) drives cellular growth 23, 24 and can be directly inhibited by AMPK through phosphorylation of its constituent protein Raptor at Ser792. 11, 24, 25, 26

Contact Form

The conversion is done automatically once the nominator, e.g. 2250, and the denominator, e.g. 3, have been inserted. The selective GAPDH inhibitors koningic acid 9 and DMF 18, 31 displayed cytotoxicity in a multitude of cancer cell lines in vitro and in vivo, including colon cancer cells, 32 breast cancer cells, 33 melanoma cells 34 and hepatocellular carcinoma. 35 Sirio CA, Tajimi K, Taenaka N, et al. A cross-cultural comparison of critical care delivery: Japan and the United States. Chest. 2002;121:539–48. Ardkhean R., Caputo D. F. J., Morrow S. M., Shi H., Xiong Y., Anderson E. A., Chem. Soc. Rev., 2016, 45, 1557

The data were derived from the Quality Indicator/Improvement Project (QIP), which is an administrative database project covering more than 400 voluntary acute-care hospitals in Japan. The project is operated by Kyoto University. The dataset is based on a Japanese-government-operated national database (Diagnosis Procedure Combination (DPC)), which includes each patient’s demographic data and diagnosis (including the main reason for hospital admission, major complications, past major diagnoses, and co-morbidities). Target patients Zhang Z. J., Zhang S. L., Yao Q. L., Feng G., Zhu M. H., Lu Z. H., Inorg. Chem. Front., 2018, 5, 370 Kemnitz K, Yoshihara K (1991) Entropy-driven dimerization of xanthene dyes in nonpolar solution and temperature-dependent fluorescence decay of dimers. J Phys Chem 95:6095–6104 Cotlet M, Goodwin PM, Waldo GS, Werner JH (2006) A comparison of the fluorescence dynamics of single molecules of a green fluorescent protein: one- versus two-photon excitation. ChemPhysChem 7:250–260Quartin AA, Campos MA, Maldonado DA, et al. Acute lung injury outside of the ICU: incidence in respiratory isolation on a general ward. Chest. 2009;135(2):261–8.

Doan PH, Pitter DRG, Kocher A, Wilson JN, Goodson T (2015) Two-photon spectroscopy as a new sensitive method for determining the DNA binding mode of fluorescent nuclear dyes. J Am Chem Soc 137:9198–9201 Deiana M, Mettra B, Martinez-Fernandez L, Mazur LM, Pawlik K, Andraud C, Samoc M, Improta R, Monnereau C, Matczyszyn K (2017) Specific recognition of G-Quadruplexes over duplex-DNA by a macromolecular NIR two-photon fluorescent probe. J Phys Chem Lett 8:5915–5920 Zhang F. F., Zhu Y. L., Lin Q., Zhang L., Zhang X. W., Wang H. T., Energy Environ. Sci., 2021, 14, 2954

Reduce 2250/3 to lowest terms

Sinufuu T, Kahnamoui K, Cook DJ, et al. Rationing critical care beds: a systematic review. Crit Care Med. 2004;32:1588–97. Guo J., Qin Y. T., Zhu Y. F., Zhang X. F., Long C., Zhao M. T., Tang Z. Y., Chem. Soc. Rev., 2021, 50, 5366 Hou Q. Q., Qi X. H., Zhen M. N., Qian H. L., Nie Y. F., Bai C. Y. L., Zhang S. Q., Bai X. Y., Ju M. T., Green Chem., 2021, 23, 119